Fig 7. Uracil transport assay.

Exponentially growing L972 (WT), KNP16 (Δfur4), UMP31 (Δura4), KNP32 (Δura4 Δpub1), and KNP38 (Δura4 Δpub1 Δfur4) cells were grown in YES medium for 12 h. Uracil uptake was monitored using [U-¹⁴C]uracil. After 1 min, cells were filtered on cellulose acetate membrane filter and washed three times. Radioactivity was measured using a liquid scintillation counter with xylene scintillator. Experiments were performed in triplicate, and error bars indicate standard deviation.