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. 2015 Nov 4;10(11):e0141796. doi: 10.1371/journal.pone.0141796

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© 2015 Nishino et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 9 — The uracil transporter Fur4 localizes to Golgi or vacuoles under normal growth conditions and shifts to the plasma membrane when nitrogen is consumed. Fur4 is also localized to the plasma membrane when the pub1 gene is deleted. Increased uracil uptake in pub1 mutants by the accumulated Fur4 localization in the membrane rescues the cell lysis phenotype. Ubiquitination of Fur4 is observed, but the mechanisms underlying Pub1 regulation of Fur4 localization remains to be determined. PM, Plasma Membrane.