Fig 5. Fluorescence in situ hybridization of a 225 bp introgressed from S. arvensis labeled with a Cy3 fluorochrome.

Panel A is the prophase stage cells from B. napus. Panels B-D are prophase stage cells from the root-tips of B. napus BC₇F₃ line (11-12-8). The top row in each panel is visualized with a filter for DAPI where DNA fluoresces blue. The bottom panel is the same cell visualized with a filter that detects Cy3 fluorescence; each cell in BC₇F₃ line (11-12-8) has two red/orange fluorescing dots (arrows) suggesting a homologous insertion. Bar = 1 μm.