Table 1. Hybrid production between Brassica napus and Sinapis arvensis: frequency of embryo regeneration and hybrid plant establishment upon direct and reciprocal crosses followed by in vitro culture and embryo rescue.
| Cross Combination | # of buds pollinated | # of siliques cultured | # of embryos excised | # of embryos germinated | # of hybrids obtained |
|---|---|---|---|---|---|
| Strategy I | |||||
| 1. B. napus X S. arvensis | 200 | - | 60 | 2 | 2 |
| 2. S. arvensis X B. napus | 200 | - | 30 | 0 | 0 |
| Strategy II | |||||
| 1. B. napus X S. arvensis | 150 | 110 | 55 | 9 | 9 |
| 2. S. arvensis X B. napus | 150 | 120 | 10 | 0 | 0 |
Strategy I: Siliques harvested 10–12 days after pollination, embryos excised and cultured on media. Strategy II: Immature siliques harvested 3–5 days after pollination, cultured on media for 2 weeks and after which embryos were harvested and cultured on media.