Table 1.

Quantitative mRNA measurement in hypothalamic nuclei obtained from laser capture microdissection (LCM).

Brain Region LCM	Q-PCR Probe	HCR:LCR	p-value
Arcuate nucleus	PC1	0.76	0.20
	PC2	1.23	0.45
	POMC	1.08	0.13
	AgRP	1.61	0.08
Perifornical lateral hypothalamus (PeFLH)	MC3R	1.56	0.20
	MC4R	7.14	0.04*
	MC5R	50.00	0.02*
	BDNF	7.14	0.05*
Paraventricular nucleus (PVN)	MC3R	33.33	0.03*
	MC4R	1.33	0.67
	MC5R	0.91	0.99
Ventromedial nucleus (VMN)	MC3R	0.75	0.40
	MC4R	1.00	0.99
	MC5R	0.60	0.26
	Sirt1	0.89	0.39
	SF1	0.87	0.37
	ADCYAP1	0.64	0.10
	BDNF	0.71	0.07
Dorsomedial nucleus (DMN)	MC3R	0.91	0.10
	MC4R	1.42	0.56
	MC5R	Low levels/undetectable	Low levels/undetectable

PC1, PC2: prohormone convertase 1 and 2; POMC: proopiomelanocortin; AgRP: agouti-related peptide; MC3R, MC4R, MC5R: melanocortin receptors 3, 4, and 5; Sirt1: sirtuin 1; SF-1: steroidogenic factor 1; ADCYAP: adenylate cyclate activating polypeptide 1 or prepro-PACAP; BDNF: brain-derived neurotrophic factor.

^*p<0.05 (HCR>LCR).