Figure 1.
Representative examples of calcium activity from barrel cortex neurons in Non-tg, WT α-syn, E57K α-syn, APP, and 3RTau Tg mice. A. Composite two photon image of the calcium indicator dye, OGB (green), and astrocyte marker, SR101 (yellow-orange). B. Respective two photon images indicating the SR101-positve structures. C. Regions of interest for each image. Neurons are numbered and indicated in green; astrocytes are in red. D. The time course for the calcium signal was extracted based on regions of interest identified in the previous panel for 1 and 3 Hz, respectively. The representative neuron number is indicated to the left of each trace. The arrows indicate the onset of the stimulus. Scale bar for photomicrographs is 10 µm.