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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: J Struct Biol. 2015 Aug 28;192(2):163–173. doi: 10.1016/j.jsb.2015.08.015

Table 1.

Phase residuals in resolution shells. Images were taken on a K2 Summit DDD camera mounted on an F20 electron microscope in super-resolution mode at a magnification of 40,410x with subsequent binning over 2 x 2 pixels and at a magnification of 15,858x without binning. The phase residuals are given for separately merging seven images from each imaging condition (40k, 2x2 binned and 16k, unbinned), and for merging of all 14 images (combined). For each resolution shell the phase residual (in degree, top number) and number of spots (bottom number) are given. The red lines indicate the resolution cut-off (~80º phase residual) and the blue line indicates the physical Nyquist frequency for the images taken at a magnification of 15,858x.

40k, 2x2 binned 16k, unbinned combined
# DMIN DMAX all IQs IQ-wght all IQs IQ-wght all IQs IQ-wght
1 1000.0 11.6 23.1 17.9 22.3 19.4 29.3 22.4
1 323 323 329 329 652 652
2 11.6 8.2 26.1 21.9 27.3 24.1 29.0 24.0
2 329 329 328 328 657 657
3 8.2 6.7 27.9 25.0 21.7 18.8 25.6 23.1
3 346 346 347 347 693 693
4 6.7 5.8 40.9 36.4 39.5 34.0 42.2 37.3
4 346 346 342 342 688 688

5 5.8 5.2 27.9 24.1 27.0 21.3 27.6 23.6
5 317 317 319 319 636 636
6 5.2 4.7 41.9 31.6 40.2 30.7 42.9 33.1
6 312 312 274 274 586 586
7 4.7 4.4 36.0 32.6 39.0 36.6 39.7 35.8
7 335 335 322 322 657 657
8 4.4 4.1 39.8 31.6 52.0 44.4 44.0 35.8
8 309 309 264 264 573 573
9 4.1 3.9 55.4 47.2 63.0 57.7 61.7 52.4
9 265 265 224 224 501 501
10 3.9 3.7 56.3 47.1 77.6 72.7 62.1 53.0
10 317 317 238 238 584 584
11 3.7 3.5 64.2 57.0 70.9 68.4 68.6 59.2
11 281 281 178 178 487 487
12 3.5 3.4 78.3 76.3 80.5 70.7 79.9 75.1
12 178 178 143 143 407 407



13 3.4 3.2 76.8 79.4 85.6 87.5 83.3 82.7
13 166 166 135 135 429 429
14 3.2 3.1 78.0 73.3 95.8 90.7 89.4 87.4
14 85 85 85 85 308 308
15 3.1 3.0 80.2 73.5 73.6 70.9 83.4 80.1
15 67 67 39 39 294 294

Overall: Phase residual 44.112 45.546 49.046
Number of spots 3976 3567 8152