Table 1.
Phase residuals in resolution shells. Images were taken on a K2 Summit DDD camera mounted on an F20 electron microscope in super-resolution mode at a magnification of 40,410x with subsequent binning over 2 x 2 pixels and at a magnification of 15,858x without binning. The phase residuals are given for separately merging seven images from each imaging condition (40k, 2x2 binned and 16k, unbinned), and for merging of all 14 images (combined). For each resolution shell the phase residual (in degree, top number) and number of spots (bottom number) are given. The red lines indicate the resolution cut-off (~80º phase residual) and the blue line indicates the physical Nyquist frequency for the images taken at a magnification of 15,858x.
40k, 2x2 binned | 16k, unbinned | combined | ||||||
---|---|---|---|---|---|---|---|---|
# | DMIN | DMAX | all IQs | IQ-wght | all IQs | IQ-wght | all IQs | IQ-wght |
1 | 1000.0 11.6 | 23.1 | 17.9 | 22.3 | 19.4 | 29.3 | 22.4 | |
1 | 323 | 323 | 329 | 329 | 652 | 652 | ||
2 | 11.6 | 8.2 | 26.1 | 21.9 | 27.3 | 24.1 | 29.0 | 24.0 |
2 | 329 | 329 | 328 | 328 | 657 | 657 | ||
3 | 8.2 | 6.7 | 27.9 | 25.0 | 21.7 | 18.8 | 25.6 | 23.1 |
3 | 346 | 346 | 347 | 347 | 693 | 693 | ||
4 | 6.7 | 5.8 | 40.9 | 36.4 | 39.5 | 34.0 | 42.2 | 37.3 |
4 | 346 | 346 | 342 | 342 | 688 | 688 | ||
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5 | 5.8 | 5.2 | 27.9 | 24.1 | 27.0 | 21.3 | 27.6 | 23.6 |
5 | 317 | 317 | 319 | 319 | 636 | 636 | ||
6 | 5.2 | 4.7 | 41.9 | 31.6 | 40.2 | 30.7 | 42.9 | 33.1 |
6 | 312 | 312 | 274 | 274 | 586 | 586 | ||
7 | 4.7 | 4.4 | 36.0 | 32.6 | 39.0 | 36.6 | 39.7 | 35.8 |
7 | 335 | 335 | 322 | 322 | 657 | 657 | ||
8 | 4.4 | 4.1 | 39.8 | 31.6 | 52.0 | 44.4 | 44.0 | 35.8 |
8 | 309 | 309 | 264 | 264 | 573 | 573 | ||
9 | 4.1 | 3.9 | 55.4 | 47.2 | 63.0 | 57.7 | 61.7 | 52.4 |
9 | 265 | 265 | 224 | 224 | 501 | 501 | ||
10 | 3.9 | 3.7 | 56.3 | 47.1 | 77.6 | 72.7 | 62.1 | 53.0 |
10 | 317 | 317 | 238 | 238 | 584 | 584 | ||
11 | 3.7 | 3.5 | 64.2 | 57.0 | 70.9 | 68.4 | 68.6 | 59.2 |
11 | 281 | 281 | 178 | 178 | 487 | 487 | ||
12 | 3.5 | 3.4 | 78.3 | 76.3 | 80.5 | 70.7 | 79.9 | 75.1 |
12 | 178 | 178 | 143 | 143 | 407 | 407 | ||
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13 | 3.4 | 3.2 | 76.8 | 79.4 | 85.6 | 87.5 | 83.3 | 82.7 |
13 | 166 | 166 | 135 | 135 | 429 | 429 | ||
14 | 3.2 | 3.1 | 78.0 | 73.3 | 95.8 | 90.7 | 89.4 | 87.4 |
14 | 85 | 85 | 85 | 85 | 308 | 308 | ||
15 | 3.1 | 3.0 | 80.2 | 73.5 | 73.6 | 70.9 | 83.4 | 80.1 |
15 | 67 | 67 | 39 | 39 | 294 | 294 | ||
| ||||||||
Overall: Phase residual | 44.112 | 45.546 | 49.046 | |||||
Number of spots | 3976 | 3567 | 8152 |