Skip to main content
. Author manuscript; available in PMC: 2016 Dec 3.
Published in final edited form as: Neuroscience. 2015 Sep 14;310:322–341. doi: 10.1016/j.neuroscience.2015.09.006

Table 5.

Mean and standard error of mean values for α4 – immunoreactivity for mice injected with P4 or oil

Spine Shaft
Group Sub-group N Membrane Cytoplasm Total Membrane Cytoplasm Total
P4 injected Resistant 6 0.48 ± 0.14 1.1 ± 0.2 0.97 ± 0.18 7.05 ± 1.75 9.8 ± 2.98 10.12 ± 3.02
Vulnerable 6 0.058 ± 0.027 0.17 ± 0.066 0.23 ± 0.17 0.76 ± 0.48 0.7 ± 0.21 0.68 ± 0.24
Sensitized 4 0.65 ± 0.14 1.28 ± 0.23 1.21 ± 0.13 8.43 ± 2.03 12.97 ± 3.48 13.29 ± 3.5
Non-sensitized 8 0.081 ± 0.031 0.31 ± 0.11 0.29 ± 0.072 1.65 ± 0.9 1.44 ± 0.58 1.46 ± 0.62
Oil injected Resistant 3 0.32 ± 0.14 0.52 ± 0.15 0.88 ± 0.32 2.74 ± 0.98 3.89 ± 1.73 3.99 ± 1.75
Vulnerable 7 0.18 ± 0.042 0.57 ± 0.16 0.78 ± 0.16 1.81 ± 0.61 3.75 ± 0.56 3.8 ± 0.57
Sensitized 3 0.17 ± 0.017 0.37 ± 0.044 0.57 ± 0.073 2.73 ± 0.99 4.34 ± 1.33 4.43 ± 1.36
Non-sensitized 7 0.24 ± 0.073 0.63 ± 0.16 0.91 ± 0.21 1.82 ± 0.61 3.56 ± 0.68 3.61 ± 0.68
Resistant All 9 0.43 ± 0.1 0.9 ± 0.17 0.94 ± 0.14 5.62 ± 1.37 7.87 ± 2.22 8.07 ± 2.26
Vulnerable All 13 0.12 ± 0.03 0.39 ± 0.1 0.53 ± 0.13 1.33 ± 0.41 2.35 ± 0.53 2.36 ± 0.54
Sensitized All 7 0.44 ± 0.12 0.89 ± 0.22 0.94 ± 0.15 5.99 ± 1.62 9.27 ± 2.6 9.49 ± 2.64
Non-sensitized All 15 0.16 ± 0.042 0.46 ± 0.1 0.58 ± 0.13 1.73 ± 0.54 2.43 ± 0.51 2.46 ± 0.53

Measures for α4 –immunoreactivity on spines are computed as the number of SIGs found per 10 spines, whether on the plasma membrane, in the cytoplasm or overall in the spine (at the plasma membrane + cytoplasmic).

Measures for α4 –immunoreactivity on dendritic shafts were computed as follows. The number of membranous SIGs for each animal was divided by the total length of the plasma membrane sampled for that animal to obtain a density value (# SIG/100 μm of plasma membrane length). The total plasma membrane length sampled was equalized across all animals (~ 85 μm). A similar measure was calculated for cytoplasmic SIG and total (at the plasma membrane + cytoplasmic) SIG where the total number of cytoplasmic and total SIGs in the dendritic shafts of an animal was divided by the total area of sampled dendritic shafts to obtain the number of SIG per unit μm2.

Red italics indicate that among the P4-injected animals, values corresponding to the resistant and sensitized animals are higher than those corresponding to the vulnerable and non-sensitized animals, respectively.

Bolded and italicized values indicate a significant difference within the P4-injected animals as described for the red italicized values with an additional significant difference between the P4-injected and corresponding oil-injected group.

Bolded values corresponding to oil-injected animals indicate these are significantly higher than the corresponding P4-injected animals.

Bolded values corresponding to overall resistant and sensitized group indicates these are significantly higher than the vulnerable or non-sensitized group as applicable.