Skip to main content
. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Cancer Prev Res (Phila). 2015 Aug 14;8(11):1036–1044. doi: 10.1158/1940-6207.CAPR-14-0464

Figure 3. Soy bread consumption and phenotypic changes in circulating MDSC populations in patients with prostate cancer.

Figure 3

Peripheral blood mononuclear cells (PBMCs) were obtained from the 25 evaluable cancer patients on Day 0 and Day 56, and stained for MDSC using flurochrome-labeled antibodies targeting CD33, HLADR and CD14 or the appropriate isotype controls. MDSC levels were evaluated by flow cytometry based on a minimum of 20,000 live events and presented as the percentage of total cells. Data were analyzed and presented as the total percentage of (A) CD33+HLA-DRnegCD14+ monocytic MDSC. Each symbol represents data from an individual patient while the horizontal lines indicate the mean within each group. (B) Representative flow cytometric dot plots validating effective depletion of MDSC from patient peripheral blood samples. Depletion of CD33+ cells results in enhanced proliferation following a 72 hour stimulation with CD3/CD28 beads in CFSE-labeled (C) CD4+ and (D) CD8+ T cells from peripheral blood of patients. Data are presented as the percentage of T cell proliferation from blood taken on Day 0 from five representative patients on study. Cryopreserved cells were thawed, CFSE-labeled, stimulated, stained for CD4 or CD8 markers and analyzed by flow cytometry.