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VP mRNA expression in the paraventricular (PVN) and supraoptic (SON) nuclei of the hypothalamus of adult Wistar rats. Cryocut coronal brain sections (16 μm) were processed and hybridized according to Dumais et al (2013) and exposed to film. The optical density of VP mRNA was measured as arbitrary units using Image J (NIH, http://rsb.info.nih.gov/ij/). Values indicate means ± SEM.
| Region | Males (n=11) | Females (n=28) |
|---|---|---|
| PVN | 80.9 ± 4.0 | 81.1 ± 2.1 |
| SON | 95.6 ± 3.1 | 98.6 ± 1.8 |
