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. 2015 Jul 5;47(12):2475–2482. doi: 10.1007/s00726-015-2020-3

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© The Author(s) 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 4 — The effect of apelin on AVIC mineralization. Human AVICs were incubated in vehicle, PCM and PCM containing 100 pM apelin for 21 days. a A representative entire plate view of the von Kossa staining method in 24-well plates. b A representative microscopic view of von Kossa method staining at a magnification of ×400 in 24-well plates. c Calcium content was reduced by apelin (*P < 0.05 vs. PCM group)