Figure 2.

Determination of transfection efficiency of pCMV6-AC-GFP vector using flowcytometry. Right: detection of transfection efficiency by flowcytometry. Transfection efficiency was maintained at 60%, 48 hr post-transfection. Left: dot blot flowcytometry after 48 hr. High efficiency of transfection with fluorescent GFP (green) in A549 cells was easily identified for 48 hr post-transfection (×100). Green fluorescence intensities (FL1-H) are indicated on the x axis, and cell counts are indicated on the y axis. The cytometric analysis was performed for 2 × 10⁴ events