Table 1.

The primer sequences (forward, reverse) which were used in the real-time RT-PCR technique

Gene	Forward (top) Reverse (bottom)	Size (bp)	Real time RT-PCR program
Nestin	5’-AACAGCGACGGAGGTCTCTA-3’ 5’-TTCTCTTGTCCCGCAGACTT-3’	220	94 °C – 20 sec, 59 °C – 30 sec, 72 °C – 30 sec 35 cycles
MAP2	5’-TCAGAGGCAATGACCTTACC-3’ 5’-GTGGTAGGCTCTTGGTCTTT-3’	321	94 °C – 20 sec, 57 °C – 30 sec, 72 °C – 30 sec 45 cycles
GFAP	5’-CCTCTCCCTGGCTCGAATG-3’ 5’-GGAAGCGAACCTTCTCGATGTA-3’	161	94 °C – 20 sec, 59 °C – 30 sec, 72 °C – 30 sec 40 cycles
GAPDH	5’-ACCACAGTCCATGCCATCAC-3’ 5’-TCCACCACCCTGTTGCTGTA-3’	452	94 °C – 20 sec, 60 °C – 30 sec, 72 °C – 30 sec 25 cycles