Table 2.

Zeta potential (mV) and mean fluorescence intensity of normal, heat treated and autoclaved E. coli and S. aureus

Treatment	Zeta potential (mV)		Fluorescence intensity (NPN uptake)
	E. coli	S. aureus	E. coli	S. aureus
Normal bacterial cell	−44.2 ± 0.50	−35.6 ± 0.54	975 ± 55.49	855 ± 60.83
Heat treatment for 10 min at 100 °C	−33.9 ± 0.81**	−35.13 ± 0.60	1248 ± 138.82**	872 ± 62.96
Heat treatment for 30 min at 100 °C	−21.1 ± 0.62**	−27.6 ± 0.54**	1543 ± 76.01**	1209 ± 70.34**

Values are expressed as mean ± SEM; (n = 6)

The respective values of Zeta potential and NPN assay of normal, heat treated and autoclaved E. coli and S. aureus

The data are the means ± the standard deviations (n = 6)

** p < 0.01. (vs. control)