Figure 2.

Assessing the effect of a deletion of mobA gene on molybdenum enzymes activities and gene expression in NTHI. (A) Enzyme activity of different molybdenum enzymes in HI2019^WT, HI2019^ΔmobA, and HI2019^ΔmobA_comp strains. Reductase activities were analyzed in crude extracts after anaerobic growth of HI2019^WT, HI2019^ΔmobA, and HI2019^ΔmobA_comp strains in sRPMI. DMSO reductase (DMSOR), nitrate reductase (NR), and MetSO reductase (MetSOR) activities were expressed as μmol of DMSO, nitrate, and MetSO reduced per min (U) per mg of proteins, respectively. Formate dehydrogenase (FDH) activity was assayed in crude extracts after microaerophilic growth and was expressed as μ mol of formate oxidized per min (U) per mg of proteins.(B) Expression of genes encoding or involved in molybdenum enzymes activities in HI2019^ΔmobA grown under aerobic, microaerophilic, and anaerobic conditions. dmsA, DMSOR; torZ, putative TMAO reductase; napA, NR; modE, activator of the transcription of genes involved in cellular molybdenum metabolism.