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. 2015 Nov 5;9:72. doi: 10.3389/fncir.2015.00072

FIGURE 5.

FIGURE 5

Bulk Regional Viral Injection permits in vivo structural imaging of OB tufted cells. (A) Single optical sections from a 2-photon z-stack of the dorsal surface of the OB of a 6 week-old CCK-IRES-Cre mouse injected with AAV1-tdTomato at P0. Numbers indicate depth in μm. Magenta brackets indicate intrabulbar-projecting tufted cell axons in the IPL at depths of 350–400 μm. (B) Same as A but different region of interest with higher magnification, illustrating tdTomato-expressing external and superficial tufted cells and lateral dendrites in the EPL. Magenta arrowheads indicate apical tufts at 50–75 μm depth, and magenta arrows indicate lateral dendrites at 175–200 μm depth.