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. 2015 Nov 5;9:72. doi: 10.3389/fncir.2015.00072

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Copyright © 2015 Cheetham, Grier and Belluscio.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Bulk Regional Viral Injection permits in vivo calcium imaging of OB tufted cells. (A) (i) Coronal OB section from a P25 CCK-IRES-Cre mouse injected with AAV1-Flex-GCaMP6f at P3. Note low resting fluorescence of GCaMP6f. (ii) Single optical section showing GCaMP6f-labeled tufted cells. (B) In vivo 2-photon images showing responses to odor stimulation (1% EB, 3 s) in two separate trials at 90 s intervals. Overlaid letters and numbers correspond to traces in C. (Ci) Normalized ΔF/F for tufted cells marked in B lower left panel. Cell A is located at the periphery of the EB-responsive glomerulus. (Cii) Normalized ΔF/F for tufted cell dendrites in ROIs marked in B lower right panel. ROIs 1–5 correspond to the EB-responsive glomerulus; ROIs 6–7 correspond to adjacent unresponsive glomeruli.