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. 2015 Nov 5;9:72. doi: 10.3389/fncir.2015.00072

FIGURE 7.

FIGURE 7

Bulk Regional Viral Injection-mediated labeling of dopaminergic neurons throughout the OB of TH-Cre mice. (A) Coronal sections at anterior, central and posterior positions along the rostrocaudal axis showing labeling of OB neurons in a P28 TH-Cre mouse injected with AAV1-Flex-GFP at P0. Blue boxed region illustrating density of labeled neurons in the GCL is shown at 2x higher magnification with increased contrast. (B) As A but for a P28 TH-Cre;ROSA-CAG-LSL-tdTomato mouse. (C) Time lapse 2-photon images (1 h intervals) of the dorsal surface of the OB of a P42 TH-Cre mouse injected with AAV1-Flex-GFP at P2. (D) Confocal images from a P56 OMP-GFP+/-;Thy1-YFP-G+/-;TH-Cre+/- mouse injected with AAV1-Flex-tdTomato at P2, showing YFP labeling of mitral cell dendrites, GFP labeling of olfactory sensory neuron axons and tdTomato labeling of DAergic neurons. (i) MIP of confocal z-stack of the dorsal surface of the OB. (ii, iii) Single optical sections showing the medial and dorsal surfaces, respectively.