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Bulk Regional Viral Injection in frontal cortex permits population labeling of cortical CCK+ neurons and their projections. (A–C) Coronal sections from a CCK-IRES-Cre mouse injected with AAV-Flex-tdTomato in the frontal cortex at P0. (A–C) are at progressively more posterior positions along the rostrocaudal axis. (D) MIPs of higher magnification confocal z-stacks showing tdTomato labeling of (i) neurons in anterior cingulate cortex and (ii) axons in the corpus callosum.
