Table 1.
Species identification of 22 isolates of Weissella species by 16S rRNA sequencing analysis and the MALDI-TOF Biotyper system using preexisting (DB 5627) and newly established databases.
| Isolate no. | Original identification results by conventional methods | 16S rRNA sequencing |
Identification by MALDI Biotyper based on database 5627 |
Identification by MALDI Biotyper based on newly developed database |
|||
|---|---|---|---|---|---|---|---|
| Results (maximal identity, %) | Accession number | Results | Score value | Results (best match) | Score value | ||
| 1∗ | Lactobacillus species | W. confusa (100) | KF245541.1 | NRI | 1.361 | – | – |
| 2∗ | Leuconostoc species | W. confusa (99.8) | EU807756.1 | NRI | 1.336 | – | – |
| 3∗ | Leuconostoc species | W. confusa (100) | GU138614.1 | NRI | 1.298 | – | – |
| 4 | Lactobacillus species | W. confusa (100) | KJ476186.1 | NRI | 1.391 | W. confusa | 2.054 |
| 5 | Lactobacillus species | W. confusa (100) | KF245541.1 | NRI | 1.448 | W. confusa | 2.073 |
| 6 | Lactobacillus species | W. confusa (100) | KF245541.1 | NRI | 1.29 | W. confusa | 2.250 |
| 7 | Lactobacillus species | W. confusa (100) | KF245541.1 | NRI | 1.39 | W. confusa | 2.243 |
| 8 | Lactobacillus species | W. confusa (100) | KF245541.1 | NRI | 1.43 | W. confusa | 2.501 |
| 9 | Lactobacillus species | W. confusa (100) | KF245541.1 | NRI | 1.338 | W. confusa | 2.376 |
| 10 | Lactobacillus species | W. confusa (100) | KJ476186.1 | NRI | 1.503 | W. confusa | 2.113 |
| 11 | Leuconostoc species | W. confusa (99.9) | KJ476186.1 | NRI | 1.303 | W. confusa | 2.395 |
| 12 | Leuconostoc species | W. confusa (100) | EU807756.1 | NRI | 1.35 | W. confusa | 2.101 |
| 13 | Leuconostoc species | W. confusa (100) | EU807756.1 | NRI | 1.362 | W. confusa | 2.04 |
| 14 | Leuconostoc species | W. confusa (99.6) | KJ476186.1 | NRI | 1.391 | W. confusa | 2.332 |
| 15 | Lactobacillus species | W. confusa (100) | GU138614.1 | NRI | 1.416 | W. confusa | 2.333 |
| 16 | Leuconostoc species | W. confusa (99.7) | GU138614.1 | NRI | 1.394 | W. confusa | 2.022 |
| 17 | Leuconostoc species | W. confusa (100) | GU138614.1 | NRI | 1.38 | W. confusa | 1.963 |
| 18 | Leuconostoc species | W. confusa (99.8) | KJ476186.1 | NRI | 1.282 | W. confusa | 2.368 |
| 19 | Leuconostoc species | W. confusa (100) | GU138614.1 | NRI | 1.413 | W. confusa | 2.302 |
| 20 | Lactobacillus species | W. confusa (100) | KJ476186.1 | NRI | 1.382 | W. confusa | 2.355 |
| 21 | Leuconostoc species | W. cibaria (100) | AB510747.1 | NRI | 1.333 | – | – |
| 22 | Leuconostoc species | W. cibaria (99.3) | AB510747.1 | NRI | 1.519 | – | – |
∗Isolates for creation of W. confusa spectra; NRI, no reliable identification.