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. 2015 Oct 22;2015:569071. doi: 10.1155/2015/569071

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Copyright © 2015 R. Rogojanu et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Example of an image of a CRC tissue section analyzed by EPSTRA. (a) K8 staining (TxRed) overlaid with DAPI (blue) including Cy2 (green) and Cy5 (white) background fluorescence; (b) epithelial mask (yellow) and stromal mask (green) as found by EPSTRA; (c) the adjacent mucosa is depicted in green contours, (d) tumor center in red, (e) invasive front 1 in yellow, and invasive front 2 in pink; (f)–(h) show the corresponding epithelial mask of (c)–(e) views. Bubbles show the region of interest (ROI) identification number and its area. Bar indicates 200 μm.