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. 2015 Nov 5;5:16077. doi: 10.1038/srep16077

Figure 1. XPO1 inhibition by Specific Inhibitor of Nuclear Export (SINE) compounds blocks EMT.

Figure 1

(A,B top panels) XPO1 inhibition reverses EMT. HMLE-snail and HMLER-snail cells were grown (50,000 cells/well in six well-plates) for 24 hrs followed by exposure to different SINE compounds and inactive analog KPT-301 (at 1 μM each) for another 24 hrs. Photomicrographs (40× magnification) showing elongated cells in the control and circular cells in the treated groups. KPT-301 (−ve control) does not induce any change in morphology of either HMLE-Snail or HMLER-Snail. Pictures representative of three independent experiments (A,B lower panel) SINE not inactive analog [150 nM] once a week for two weeks disrupt HMLE spheroid formation. Spheroids are representative of three independent experiments (C,D) Growth inhibition (MTT assay 72 hrs) by SINE compounds selinexor, KPT-185 (+ve control) not by inactive analog KPT-301. Graphs representative of three independent experiments with six replicates per dose presented. (E,F) SINE not inactive analog induce apoptosis in HMLE-snail and HMLER-snail cells. 50,000 cells were grown in six well plates in triplicate overnight followed by exposure to (150 nM) of SINE and –ve control KPT-301 for 72 hrs followed by Annexin V FITC analysis (according to manufacturer’s protocol BD Biosciences, USA). Apoptosis images representative of three independent experiments. (G,H) Apoptosis analysis in HMLE-snail and HMLER-snail by Histone DNA ELISA (Roche) under similar treatment conditions. **p < 0.01 when compared to control.