Figure 3.

Female-specific lethality of DH1 (A) and DH2 (B) under different tetracycline conditions. Containers were set with 8 pairs of adults and the number of third instar larvae (L3), pupae and adult male and female offspring counted. ++W: water with 100 μg/mL tetracycline from day 1 (D1) to D8; +−W: water with limited tetracycline (1 μg/mL for DH1 and 3 μg/mL for DH2) from D1 to D2, then switched to water without tetracycline from D3 to D8; +M: ground meat (larval diet) with 100 μg/g tetracycline; -M: meat without tetracycline. Mean and standard error are shown from three replicate experiments.