Figure 5. Female-specific lethality of All-in-one lines.

(A) Schematic illustration of the All-in-one construct, which contains both driver and effector cassettes in the pB[Lshsp83-RFPex] vector. (B) Female-specific lethality of heterozygotes. 8 homozygous 1#Brt or 7# (X-linked) virgin females were crossed with 8 wild type males and their offspring were raised on diet without tetracycline. The number of L3, pupae, adult male and female offspring from each cross were counted. Female-specific lethality of homozygous lines 1#Brt (C) and 7# (D). Parental generation feeding regimen: +W: water with 100 μg/mL tetracycline from D1 to D8; −W: water with no tetracycline from D1 to D8. +M is 100 μg/g tetracycline added to the larval diet (meat), −M is without tetracycline. Mean and standard error are shown from three replicate experiments.