Table 2. Effect of hydroxylamine on temperature-dependent hyperactivation of human spermatozoa.

Treatment	T(°C)	FD	%HYP
No hydroxylamine	34	1.25 ± 0.01	13 ± 2
	37	1.17 ± 0.01*	4 ± 1*
With hydroxylamine	34	1.16 ± 0.01	2 ± 1
	37	1.15 ± 0.02	1.9 ± 0.5

The motility parameters FD and percentage of hyperactivated spermatozoa (%HYP) were consecutively analysed at 34 °C and 37 °C for 1 min periods under the illumination of a bright-field microscope in the presence and absence of hydroxylamine (5 mM). Each of the values shown is the mean ± SEM of 12 determinations. *P < 0.0001 according to two-way ANOVA for the difference from 34 °C.