Table 2. Effect of hydroxylamine on temperature-dependent hyperactivation of human spermatozoa.
Treatment | T(°C) | FD | %HYP |
---|---|---|---|
No hydroxylamine | 34 | 1.25 ± 0.01 | 13 ± 2 |
37 | 1.17 ± 0.01* | 4 ± 1* | |
With hydroxylamine | 34 | 1.16 ± 0.01 | 2 ± 1 |
37 | 1.15 ± 0.02 | 1.9 ± 0.5 |
The motility parameters FD and percentage of hyperactivated spermatozoa (%HYP) were consecutively analysed at 34 °C and 37 °C for 1 min periods under the illumination of a bright-field microscope in the presence and absence of hydroxylamine (5 mM). Each of the values shown is the mean ± SEM of 12 determinations. *P < 0.0001 according to two-way ANOVA for the difference from 34 °C.