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. 2015 Nov 5;5:16228. doi: 10.1038/srep16228

Figure 1. E5 inhibits MS-5 mediated-protection and sensitizes leukemia cells to different chemotherapies.

Figure 1

(A) E5 enhances vincristine, cisplatin or 10-hydroxy camptothecin-induced apoptosis in HL-60 cells co-cultured with MS-5 cells. HL-60 cells were treated with drugs alone, or drugs in combination with E5 in the absence or presence of MS-5 cells for 48 h. Apoptotic cells were detected by Annexin V flow cytometry. (B) E5 enhances 10-hydroxy camptothecin-induced apoptosis in multiple AML cell lines including NB4, THP-1 and U937 cells co-cultured with MS-5 cells for 48 h. (C) After continuous combination treatment, vincristine drastically reduces HL-60 cell viability. HL-60 cells growing on MS-5 cell layer were treated with vincristine alone or combined with E5 for 10 days and cell viability was detected using trypan blue every day. Data are presented as mean ± SD (n = 3). The * represents significant difference between two groups (*p < 0.05, **p < 0.01).