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. 2015 Nov 5;5:16269. doi: 10.1038/srep16269

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Copyright © 2015, Macmillan Publishers Limited

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(A,B) Expression of FLNa was assessed by intracellular flow cytometry coupled to extracellular flow cytometry in CD11c^hiCD4⁺CD8α⁻ (CD4⁺; green), CD11c^hiCD4⁻CD8α⁺ (CD8α⁺; pink) and CD11c^hiCD4⁻CD8α⁻ (DN; blue) spleen cDC subsets from Mx1-Cre (+/+) and Mx1-Cre;ASB2^fl/fl (−/−) mice that have received poly(I·C). Filled histograms show expression of FLNa in cDC subsets of control mice whereas unfilled histograms show expression of FLNa in cDC subsets of Mx1-Cre;ASB2^fl/fl mice that have received poly(I·C) (sample size: +/+ = 3; −/− = 4). One representative experiment out of three is presented. (C) Relative expression of FLNa mRNA assessed by RT-qPCR in CD4⁺, CD8α⁺ and DN cDC subsets in spleen of Mx1-Cre (+ /+ ) and Mx1-Cre;ASB2^fl/fl (− /− ) mice that have received poly(I·C) (sample size: +/+ = 6; −/− = 6). (D) Expression of FLNa was assessed by intracellular flow cytometry coupled to extracellular flow cytometry in CD4⁺, CD8α⁺ and DN cDCs isolated from mesenteric lymph nodes of ASB2^+/+ mice (sample size = 5). One representative experiment out of three is presented.