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. 2015 Nov 5;5:16269. doi: 10.1038/srep16269

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Copyright © 2015, Macmillan Publishers Limited

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CD11c⁺ spleen cells of ASB2^+/+ mice were plated into transwell inserts and allowed to chemotax towards medium containing or not SDF1α, as indicated. After 2 hours at 37 °C, migrated cells were recovered from the wells and quantified by flow cytometry together with input cells. For each cDC subset, the ratio between the percentage of migrated cells and the percentage of input cells as well as SEM of three independent experiments are shown (sample size = 16). The line indicates the 1:1 ratio achieved where all cDC subsets migrate with equal efficiency.