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. 2015 Nov 5;5:16269. doi: 10.1038/srep16269

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This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) Experimental design. (B) Experimental outline of ASB2 deletion and generation of GM-CSF BMDCs. (C) Expression of CD11c and CD86 at the cell surface of ASB2α^−/− and ASB2α^+/+ BMDCs. (D) Expression of FLNa, FLNb, ASB2α, α-actinin 1 was analyzed by western blot using 10-μg aliquots of protein extracts of ASB2α^−/− and ASB2α^+/+ BMDCs from three independent experiments and 3-μg aliquots of cell extracts of HeLa cells transfected with a mouse ASB2α expression vector (ASB2α) or mock-transfected (−). The drawing in panel B is from P.G.L.