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. 2015 Nov 5;5:16271. doi: 10.1038/srep16271

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Adult rat testes were topically injected with a synthetic peptide F5 to induce the impairment of spermatogenesis. (A) Immunoblots of Slc15a1 in 0 h, 12 h, 1 d, 2 d, 3 d, 4 d and 5 d testis lysates following F5 treatment, with actin as a loading control. (B) Histogram summarizing results after normalizing each data point against actin. The value at 0 h was arbitrarily set as 1. Bars are means ± sd; n = 3. 1-way ANOVA followed by Dunnett’s test.