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. 2015 Nov 5;5:16271. doi: 10.1038/srep16271

Figure 4. The in vivo RNAi of Slc15a1 in adult rat testes.

Figure 4

(A) The regimen used in this study. Briefly, the right testes of rats (n = 9) were injected with 100 nM Slc15a1 siRNA duplexes, while the left ones were treated with 100 nM non-targeting control siRNA on day 0. Two days later (day 2), 3 rats were killed and their testes were removed and lysed for immunoblotting to examine the silencing effect of Slc15a1 RNAi. F5-peptide at 320 μg/testis was then injected to each testis of the remaining 6 rats. Three days thereafter (day 5), 3 rats were sacrificed and their testes were harvested for immunoblotting and HE staining, while the remaining 3 rats were subject to BTB assay. (B) Representative immunoblots illustrating an ∼90% and ∼ 75% knockdown of Slc15a1 in the testes on day 2 and day 5 respectively after transfection with siRNA duplexes. C) Densitometric analysis of Slc15a1 immunoblotting data normalized against actin with the control arbitrarily set at 1. Each bar is the mean ± SD of three experiments, **P < 0.01.