Skip to main content
. Author manuscript; available in PMC: 2016 Nov 15.
Published in final edited form as: J Neuroimmunol. 2015 Sep 4;288:25–33. doi: 10.1016/j.jneuroim.2015.08.020

Figure 1. Monocyte Identification and Phenotype Gating Strategy.

Figure 1

Top row: Singlets were identified using forward scatter (FSC) height (H) and area (A). Dead cells, staining positive for Yellow Reactive Amine Dye (YARD), were excluded and PBMCs were identified by size and granularity (side scatter (SSC) vs FSC). Middle row: CD3 positive cells (lymphocytes) were excluded, along with CD19-20-56 positive cells (B cells and natural killer cells). Cells positive for HLA-DR were identified as monocytes. Bottom row: HLA-DR positive cells were subset according to CD14 and CD16 expression. Mono1 are characterized as being CD14++CD16−; Mono2, CD14++CD16+; Mono3hi, CD14+CD16+; Mono3lo, CD14loCD16+ and Mono4, CD14+CD16−.