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. 2015 Feb 9;34(42):5395–5405. doi: 10.1038/onc.2014.468

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Identification of HACE1 as a HER2/neu cooperating tumor suppressor gene. (a) VBIM soft agar screen design. (b) Typical colony formed in soft agar. Bright-field and green fluorescent protein (GFP) filter images taken at × 10 magnification. (c) Mapping of viral integration site of clone 1.10 to murine HACE1 Exon22. (d) RT–PCR for HACE1 on cDNA from parental mouse mammary tumor virus-neu mouse mammary epithelial cells and clone SD1.10. (**P< 0.001 between groups, Student's t-test).