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. 2015 Feb 9;34(42):5395–5405. doi: 10.1038/onc.2014.468

Figure 4.

Figure 4

Rac inhibition reverses phenotypic effects of HACE1 loss. (a) Rac1-GTP levels in MCF12A shHACE1 (1 and 2) knockdown and MCF12A shNSC cells as determined by Rac1-GTP pull-down. Cells were stimulated for 30 min with 100 ng/ml EGF and 10 ng/ml HRG in the presence of 25 μM EHT1864 or vehicle after overnight serum starvation. (b) Soft agar colony formation of MCF12A shHACE1 (1 and 2) and MCF12A shNSC cells in the presence of 50 μM EHT1864 or vehicle (**P<0.001 between groups, Student's t-test). Data are expressed as mean±s.e.m. of three separate experiments. (c) In vitro migration (20 h) of MCF12A shHACE1 (1 and 2) and MCF12A shNSC cells as determined by Boyden chamber in the presence of 25 μM EHT1864 or vehicle. A quantity of 100 ng/ml EGF and 10 ng/ml HRG was used chemoattractant (*P<0.01 between groups, Student's t-test). Data are expressed as mean±s.e.m. of three separate experiments.