Table 1.
Detection of Bacterial Small Molecules in Mouse and Human Samples
| Sample | Molecule Targeted | Detection Among Mice, by Tuberculosis Disease Status (%) |
||
|---|---|---|---|---|
| Infected | Uninfected | P Value | ||
| (a) | ||||
| Serum | Mycobactin T | 7/7 (100) | 0/6 (0) | <.001 |
| Carboxymycobactin | 1/7 (0) | 0/6 (0) | 1.00 | |
| TbAd | 3/7 (43) | 0/6 (0) | .192 | |
| Lung | Mycobactin T | 7/7 (100) | 0/6 (0) | <.001 |
| Carboxymycobactin | 0/7 (14) | 0/6 (0) | 1.00 | |
| TbAd | 7/7 (100) | 0/6 (0) | <.001 | |
| Sample | Molecule Targeted | Detection in Patient Sputum Samples, by Tuberculosis Disease Status (%) |
||
| Infected | Uninfected | P Value | ||
| (b) | ||||
| Sputum | Mycobactin T | 9/10 (90) | 0/10 (0) | .00058 |
| Carboxymycobactin | 0/10 (0) | 0/10 (0) | 1.00 | |
| TbAd | 6/10 (60) | 0/10 (0) | .011 | |
| Correlation Coefficient | P Value | |||
| (c) | ||||
| Mycobactin T | ||||
| AFB smear microscopy score | 0.8739 | .0024 | ||
| MGIT TTP time | −0.2970 | .4069 | ||
| GeneXpert grade | 0.5919 | .0952 | ||
| TbAd | ||||
| AFB smear microscopy score | 0.8587 | .0095 | ||
| MGIT TTP time | −0.5253 | .0989 | ||
| GeneXpert grade | 0.8980 | .0079 | ||
| Detection in Patient Cerebrospinal Fluid Samples, by Culture-Confirmed Meningitis Diagnosis (%) |
||||
| Molecule Targeted | Tuberculosis | Cryptococcal | P Value | |
| (d) | ||||
| Mycobactin T | 10/14 (53) | 0/12 (0) | .0002 | |
| TbAd | 1/14 (7) | 0/12 (0) | 1.00 | |
| Sample | Detection of M. tuberculosis in Patient Lymph Node Samples, Fraction Positive (%) |
P Value | ||
| Detection Method | Infected | Uninfected | ||
| (e) | ||||
| Lymph node | Any BSMs | 2/5 (40) | 0/5 (0) | .44 |
| AFB smear | 1/5 (20) | 0/5 (0) | 1.00 | |
| GeneXpert | 5/5 (100) | 0/5 (0) | .008 | |
| Culture | 5/5 (100) | 0/5 (0) | .008 | |
| Blood | Any BSMs | 1/5 (20) | 0/5 (0) | 1.00 |
| GeneXpert | 1/5 (20) | 0/5 (0) | 1.00 | |
| Culture | 0/5 (0) | 0/5 (0) | 1.00 | |
Abbreviations: AFB, acid-fast bacilli; BSMs, bacterial small molecules; MGIT, mycobacterial growth indicator tube; TbAd, tuberculosinyladenosine; TTP, time to positivity.