This work is licensed under a Figure 4.
The flow chart and representative pictures of the endometriotic animal model. (A) Fifty NOD–SCID female mice transplanted with human normal endometrium were separated into six groups: mice treated with E2 plus positive β-catenin siRNA or scrambled siRNA and saline solution control after a 10-day incubation; mice treated with E2 plus positive β-catenin siRNA or scrambled siRNA and saline solution control after a 21-day incubation. (B and C) Representative pictures of the implanted fragments of endometrial tissues into the peritoneal cavity of the NOD–SCID mice after transplantation of normal endometrium. The arrows indicate the implanted endometrial tissues. (D) The murine lesions were examined by histopathology. (E) Western blotting analysis of non-phosphorylated β-catenin and ESR1 expression of pre-transplanted tissues and endometriotic lesions obtained from mice model (a 10-day incubation). (F) Western blotting analysis of non-phosphorylated β-catenin and ESR1 expression of pre-transplanted tissues and endometriotic lesions obtained from mice model (a 21-day incubation). H&E, hematoxylin and eosin stain.