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Reduced or absent secretion of new rADAMTS13 mutants. (A) Representative Western blot analysis of rADAMTS13 proteins in transfected HEK293T cells supernatant. Lane 1: wild-type (WT) rADAMTS13; lane 2: mutant rADAMTS13; lane 3: untransfected cells. (B) Error bars are mean±SD secretion levels (n=3 determinations) for each mutant rADAMTS13 protein reported as a percentage of WT-rADAMTS13. rADAMTS13, recombinant a disintegrin and metalloprotease with thrombospondin motifs 13.
