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. 2015 Aug 15;5(9):2569–2589.

Figure 8.

Figure 8

Gas1 blocks miR-148a induced autophagy in Hepatic stellate cells. (A-C) LX-2 and T-6 cells were transiently co-transfected with negative control, the miR-148a mimic or the miR-148a mimic together with Gas1. The protein expression of autophagy-related LC3-II and p62 were significantly blocked by Gas1 through immunoblotting (A). The bar graphs showed the fold change of the expression level of LC3-II and p62 (B and C). GAPDH was used as loading control. (D-F) LX-2 and T-6 cells were transfected with the miR-148a mimic to induce autophagy for 48 h, followed by treated with negative control, siRNA Control or Gas1 siRNA. The protein expression of LC3-II and p62 was increased by Gas1 siRNA remarkably by immunoblotting (D). Quantitative analysis of LC3-II and p62 was shown in histograms (E and F). Results are expressed as the means ± SD from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, Unpaired student’s t-test.