Figure 9.

MiR-148a inhibits proliferation and promotes apoptosis in LX-2 and T-6 cells. A and B: MTT assay were performed on LX-2 and T-6 cells. Cells were transiently transfected with the miR-148a mimic or the inhibitor. The number of viable cells was evaluated as the value of the absorbance at 490 nm with a reference wavelength of 630 nm. Values of optical density (OD) are expressed as means ± SD (n = 3). C: Apoptosis in HSCs was determined by annexin V staining and detected by flow cytometry after 48 h of treatment. The miR-148a mimic induced early apoptosis (Lower Right region) and late apoptosis (Upper Right region) in LX-2 and T-6 cells lines. Images are representative of three independent experiments.