Skip to main content
. 2015 Aug 15;5(9):2730–2744.

Figure 4.

Figure 4

MAP2K1 controlled negative feedback regulation of miR-449a expression through c-Jun. A. miR-449a expression was detected by real-time PCR after MAP2K1 overexpression in NL9980 or knockdown in L9981 and A549. B. Three AP-1 binding sites in miR-449a promoter. C. Point mutation report gene test discovered only AP1-2 can affect MAP2K1 regulation of miR-449a promoter. D. Knockdown of c-Jun expression in NL9980 blocked MAP2K1 over-expression induced miR-449a upregulation. E and F. Over-expression of c-Jun inhibited miR-449a downregulation mediated by MAP2K1knockdown in L9981 and A549. The data is shown as the means ± SD. Student’s t test, **P<0.01.