Figure 5.

The histone H3K27me3 level of miR-449a promoter, which is mediated by SUZ12, correlates with metastasis capability in lung cancer cell lines. A. MiR-449a is located in the CDC20 gene. B. The histone H3K27me3 but not H3K9me3 level at miR-449a promoter is significantly higher in highly metastatic lung cancer cell line L9981 and A549, than low metastatic lung cancer cell line NL9980, which can be down-regulated by SUZ12 siRNA. C. The western blot assay confirmed SUZ12 expression interference. D. The histone H3K27me3 marks at miR-449a promoter was reduced after histone methyltransferase was disturbed in L9981 cells. E. MiR-449a expression was remarkably increased along with SUZ12 interference in L9981 and A549 cells. The data is shown as the means ± SD. Student’s t test, **P<0.01.