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. 2014 Jun 20;1:52–58. doi: 10.1016/j.bbacli.2014.06.002

Fig. 1.

Fig. 1

Gating strategy for the enumeration of viable CD45+[CD3/CD19/CD20/CRTH2]−CD16−CD115−CD11b+CD34+ fibrocytes in the whole blood. Absolute counting beads were successfully resolved from cells and gated for counting. The representative assay was performed with the fresh blood sample of a patient with treatment-resistant asthma.

7-AAD, 7-amino-actinomycin-D; FSC, forward scatter; PB, Pacific Blue; SSC, side scatter.