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. 2015 May 25;17(12):1578–1588. doi: 10.1093/neuonc/nov060

Fig. 4.

Fig. 4.

Positive modulation of low-molecular-weight MAP2 by PKA activation. (A) C6 glioma cells were seeded at a density of 5 × 105, incubated with PKA activators (1 mM dbcAMP, 10 ng/mL cholera toxin, and 30 μM forskolin) for 48 hours, and Western blotting was then used to evaluate the protein level of low-molecular-weight MAP2. C6 (B), DBTRG-05MG (C), and A172 (D) cells were seeded at a density of 5 × 105, pretreated with 2 μM of PKA inhibitor KT-5720 for 2 hours before 1 mM dbcAMP treatment for an additional 48 hours to evaluate the protein level of MAP2. C6 glioma cells were seeded at a density of 5 × 104, transfected with 50 nM si-PKAcα oligonucleotides for 24 hours, and then treated with or without 1 mM dbcAMP for an additional 48 hours to evaluate the morphological alterations (E) and MAP2 expression (F) (original magnification: × 200; scale bar: 100 μm). Antibodies were purchased from Cell Signaling Technology (#4542, #8707, #4782).