Figure 6. The ITT promotes plasma cell generation.

Animals from Fig. 5 were sacrificed on day 22 of the recall response and the number of antibody forming cells (AFCs) was determined by ELISPOT assays. (a) Representative wells of IgG1^a-producing (upper photo) and IgG1^b-producing (lower photo) cells from the same ITT-heterozygous animal are shown. Frequencies of AFCs per one million cells from bone marrow (BM; b) and spleens (c) are shown. (d) The ratio of IgG1^a to IgG1^b AFCs in the spleens (Sp) and BM was calculated for each animal. Error bars represent mean±s.e.m. of three (wt/wt) and five (wt/YF) analyses, respectively. Statistical significance was determined by Mann–Whitney test. *P<0.05.