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. 2015 Oct 13;6:8575. doi: 10.1038/ncomms9575

Figure 7. The ITT promotes T-cell-independent reactivation of IgG-switched memory B cells.

Figure 7

(a) Experimental outline. Female wild-type and ITT-mutant mice (seven animals per genotype) were repeatedly immunized with purified gB with or without alum as specified at the indicated time points. Splenocytes depleted of T cells and purified with anti-CD19 magnetic beads were adjusted to contain 200 mIgG1-positive, gB-specific cells and were injected into 10 Rag1-deficient mice. Seven days later recipient animals were challenged with 0.5 μg of gB-containing virus-like particles (VLPs) and sera were collected at the indicated time points. (b) Frequencies of gB-binding mIgG1-positive B cells in donor mice before transfer. Titres of gB-specific IgG1 (c) and IgG2c (d) in sera of Rag1-deficient recipient mice after challenge with VLPs. Statistical significance was determined by Mann–Whitney test. *P<0.05, **P<0.01, ***P<0.001. Data are representative of three independent experiments.