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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: J Thorac Oncol. 2015 Nov;10(11):1648–1652. doi: 10.1097/JTO.0000000000000665

Figure 1.

Figure 1

ALK break-apart FISH utilizes DNA probes that hybridize to the 3′ (red signal) and 5′ (green signal) regions of the common fusion breakpoint in ALK. Rearrangement may be identified by two variant FISH patterns – splitting of signals and 5′ deletion. A, ALK rearrangement is identified by splitting of the red and green signals in both nuclei in this field. B, Rearrangement is identified by a single red signal (red arrow) with loss of the 5′ green signal in one of the four nuclei observed. An additional three nuclei demonstrate overlapping of the 3′ and 5′ probes, reflecting wildtype ALK.