Inhibition of anthocyanin biosynthesis in fah1 mutants is dependent on the Mediator subunits MED5a and MED5b. A, Soil-grown wild-type and fah1 plants 3 weeks after planting. B, Representative HPLC chromatograms at 525 nm of seedlings after the induction of anthocyanin biosynthesis with Suc stress. For clarity, chromatograms from wild-type and med5a/5b samples are overlaid at top and those from fah1 and med5a/5b fah1 samples are overlaid at bottom. C, Quantification by HPLC of anthocyanins in Suc-stressed seedlings. Each bar shows the mean of three biological replicates for each genotype, with error bars indicating sd.