Figure 7.

ROS contributes positively to the susceptibility to B. cinerea of tomato fruit. A, Wild-type (WT) and AtMYB12 tomato fruit have lower ability to restrict the ROS burst compared with Del/Ros1 and Indigo tomatoes. 3,3′-Diaminobenzidine staining of H₂O₂ is shown between 24 and 36 h after inoculation of B. cinerea on different lines. Wound-only fruit were used as controls. Bars = 1 mm. B, B. cinerea infection of pretreated fruit. Fruit were pretreated with water, 50 µm diphenyleneiodonium chloride (DPI), and 100 units mL⁻¹ Glc oxidase + 1% Glc (GO+G) before B. cinerea infection. Lesion diameter was measured at 3 dpi. Error bars show se (n = 10). *, P < 0.05; and **, P < 0.01 indicate significant differences compared with the water treatment of the same genotype.