Figure 3.
H1.3 plays a critical role in developmental and physiological responses of Arabidopsis to environmental stresses. A, Relative expression of H1.3 (RT-qPCR) after 17 d of low-light, drought, and drought in low-light conditions. RT-qPCR measurements were performed for at least three replicates and were normalized to the expression of UBC. B and C, Effects of low-light/drought treatment on relative leaf water content (RWC; B) and absolute leaf water content (WC; C). The differences between RWC and WC values marked with different letters are statistically significant (P < 0.05, Tukey’s honestly significant difference test). D, Diagrammatic representation of the phenotype (adult plant morphology and size) in control conditions and in response to drought under limited light conditions of the wild type (WTLer), h1.3, and complemented h1.3 mutant (h1.3/H1.3). Scales are as shown. E, Venn diagram showing the number of genes with altered expression in response to low-light/drought conditions in the wild type (red), h1.3 (blue), and both genotypes (yellow). The expression pattern of selected genes was verified by RT-qPCR (Supplemental Figs. S9 and S10).