Figure 1.
Temperature responses of Rubisco kinetic parameters in S. viridis compared with corresponding values from the literature normalized to S. viridis at 25°C. The Vcmax (A), Vomax (B), KC (C), and KO (D) were determined using membrane inlet mass spectrometry on crude leaf extracts of S. viridis at pH 7.7 (at 25°C). The solid lines are the modeled temperature responses from this report (Table I), with the 95% confidence intervals shaded in gray. Dashed lines are temperature responses from previous reports normalized to S. viridis at 25°C (Badger and Collatz, 1977; Jordan and Ogren, 1984; Bernacchi et al., 2001, 2002; Walker et al., 2013). Black circles are the means of four technical replicates ± se. P values refer to the significance of the temperature response from zero, and adjusted R2 values describe the amount of variation in the measured parameter explained by the model.